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n terminal myc ddk tag  (OriGene)


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    OriGene n terminal myc ddk tag
    N Terminal Myc Ddk Tag, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+terminal+myc+ddk+tag/ITPRID2+(NM_006751)+Human+Tagged+ORF+Clone/pm38786982-154-37-40
    Average 92 stars, based on 2 article reviews
    n terminal myc ddk tag - by Bioz Stars, 2026-10
    92/100 stars

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    other:

    Article Title: KRAP tethers IP 3 receptors to actin and licenses them to evoke cytosolic Ca 2+ signals
    Article Snippet: Plasmids encoding the following proteins were from: LifeAct-mCherry-N1 (Addgene #40908) , LifAct-7-iRFP670 (Addgene #103032) , human KRAP with an N-terminal Myc-DDK tag (OriGene, Rockville, MD, USA, #RC205550), mCherry-keratin (Addgene #55066) and mCherry-vimentin-N-18 (Addgene #55158, deposited by Michael Davidson, Florida State University, FL, USA).

    Membrane:

    Article Title: KRAP regulates mitochondrial Ca2+ uptake by licensing IP3 receptor activity and stabilizing ER-mitochondrial junctions.
    Article Snippet: Imaging dishes were from Cellvis (Gerasdorf bei Wein, Austria). .. Plasmids encoding the following proteins were used: TOM20 [translocase of outer (mitochondrial) membrane 20, also known as TOMM20] tagged to GFP (TOM20–GFP) (Prole and Taylor, 2019a), Mito-R-GECO1 (Addgene, #46021) (Wu et al., 2013), human KRAP with an N-terminal myc-DDK tag (OriGene, Rockville, MD, USA, #RC205550) and siRNA-resistant human KRAP with an N-terminal myc-DDK tag (Thillaiappan et al., 2021). .. The following antibodies were used for western blotting (WB), immunocytochemistry (IC), or PLAs: anti-KRAP (rabbit polyclonal; ProteinTech, Manchester, UK, #14157-1-AP, RRID:AB_2195472; WB 1:1000), ChromoTek GFP-Booster ATTO488 (nanobody; Chromotek, Planegg-Martinsried, Germany #gba488; RRID: AB_2631386; IC 1:500), anti-MCU (rabbit polyclonal; Thermo Fisher Scientific, PA5-109304, RRID: AB_2854715; WB 1:1000), anti-β-actin (mouse monoclonal; Cell Signaling Technology, Leiden, Netherlands, #8H10D10, RRID:AB_2242334; WB 1:20,000), IRDye 680RD goat anti-mouse IgG secondary antibody (LI-COR, 926-68070, RRID:AB_10956588; WB 1:10,000), horseradish peroxidase (HRP)-conjugated sheep anti-rabbit IgG H&L (Abcam, ab6795, RRID: AB_955446; WB 1:10,000), Alexa Fluor 568-conjugated goat anti-rabbit IgG (H+L) (Thermo Fisher Scientific, A-11011, RRID:AB_143157; WB 1:10,000), Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) (Thermo Fisher Scientific, A-11008, RRID:AB_143165; WB 1:10,000), Alexa Fluor 568-conjugated goat anti-mouse IgG (H+L) (Thermo Fisher Scientific, A21235, RRID:AB_2535804; WB 1:10,000), anti-VDAC1 (Abcam, ab14734, RRID:AB_443084; PLA 1:400), anti-GFP (Abcam, ab290, RRID: AB_303395; PLA 1:200, WB 1:1000) and anti-TOM20 (Abcam, ab56783, RRID:AB_945896;WB1:1000).



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    (a) Histological alterations in cardiac tissue of transplanted patient. Hematoxylin and eosin (H&E) staining of heart explants from the transplanted case shows sarcomere disarray, with reduction of myofibrils, cardiomyocytes with irregular nuclear morphology (magnification × 40). Masson's trichrome stain revealed a development of collagen fibrillogenesis in peripheral and perinuclear areas and the rupture of myocardial fibers not observed in the heart of a control subject. Immunohistochemical analysis of the cardiac muscle show an accumulation of Filamin C aggregates (magnification × 40). (b) Expression of wild-type (WT) and mutant Filamin C proteins in transfected cells. Western blot analysis, using a flagged antibody, show high expression levels of Filamin C in the WT, with a reduction in the expression levels in the p.(F106L) mutant and a complete absence of protein expression in the p.(R991*) form. (c) Western blot analysis, using antibodies directed to the C and N terminus of Filamin C showing that no protein was produced using both variants. (d) Subcellular distribution of ectopically expressed Filamin C. Expression of WT and mutants in rat cardiac myoblast was analyzed by immunofluorescence and confocal microscopy. Filamin C expression was detected using an <t>anti-DDK</t> antibody (green), actin fibers were stained with phalloidin (red) and nuclei were counterstained with DAPI (4',6-diamidino-2-phenylindole) (magnification × 100). Expression of <t>mutant</t> <t>FLNC</t> clones resulted in the formation of perinuclear protein aggregates that were not observed in WT FLNC. The full colour version of this figure is available at European Journal of Human Genetics online.
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    (a) Histological alterations in cardiac tissue of transplanted patient. Hematoxylin and eosin (H&E) staining of heart explants from the transplanted case shows sarcomere disarray, with reduction of myofibrils, cardiomyocytes with irregular nuclear morphology (magnification × 40). Masson's trichrome stain revealed a development of collagen fibrillogenesis in peripheral and perinuclear areas and the rupture of myocardial fibers not observed in the heart of a control subject. Immunohistochemical analysis of the cardiac muscle show an accumulation of Filamin C aggregates (magnification × 40). (b) Expression of wild-type (WT) and mutant Filamin C proteins in transfected cells. Western blot analysis, using a flagged antibody, show high expression levels of Filamin C in the WT, with a reduction in the expression levels in the p.(F106L) mutant and a complete absence of protein expression in the p.(R991*) form. (c) Western blot analysis, using antibodies directed to the C and N terminus of Filamin C showing that no protein was produced using both variants. (d) Subcellular distribution of ectopically expressed Filamin C. Expression of WT and mutants in rat cardiac myoblast was analyzed by immunofluorescence and confocal microscopy. Filamin C expression was detected using an anti-DDK antibody (green), actin fibers were stained with phalloidin (red) and nuclei were counterstained with DAPI (4',6-diamidino-2-phenylindole) (magnification × 100). Expression of mutant FLNC clones resulted in the formation of perinuclear protein aggregates that were not observed in WT FLNC. The full colour version of this figure is available at European Journal of Human Genetics online.

    Journal: European Journal of Human Genetics

    Article Title: Congenital dilated cardiomyopathy caused by biallelic mutations in Filamin C

    doi: 10.1038/ejhg.2016.110

    Figure Lengend Snippet: (a) Histological alterations in cardiac tissue of transplanted patient. Hematoxylin and eosin (H&E) staining of heart explants from the transplanted case shows sarcomere disarray, with reduction of myofibrils, cardiomyocytes with irregular nuclear morphology (magnification × 40). Masson's trichrome stain revealed a development of collagen fibrillogenesis in peripheral and perinuclear areas and the rupture of myocardial fibers not observed in the heart of a control subject. Immunohistochemical analysis of the cardiac muscle show an accumulation of Filamin C aggregates (magnification × 40). (b) Expression of wild-type (WT) and mutant Filamin C proteins in transfected cells. Western blot analysis, using a flagged antibody, show high expression levels of Filamin C in the WT, with a reduction in the expression levels in the p.(F106L) mutant and a complete absence of protein expression in the p.(R991*) form. (c) Western blot analysis, using antibodies directed to the C and N terminus of Filamin C showing that no protein was produced using both variants. (d) Subcellular distribution of ectopically expressed Filamin C. Expression of WT and mutants in rat cardiac myoblast was analyzed by immunofluorescence and confocal microscopy. Filamin C expression was detected using an anti-DDK antibody (green), actin fibers were stained with phalloidin (red) and nuclei were counterstained with DAPI (4',6-diamidino-2-phenylindole) (magnification × 100). Expression of mutant FLNC clones resulted in the formation of perinuclear protein aggregates that were not observed in WT FLNC. The full colour version of this figure is available at European Journal of Human Genetics online.

    Article Snippet: Directed mutagenesis of full-length Filamin C A full-length cDNA clone for FLNC ( {"type":"entrez-nucleotide","attrs":{"text":"NM_001458.4","term_id":"188595685","term_text":"NM_001458.4"}} NM_001458.4 ) with an N-terminal Myc-DDK-Tag was obtained from OriGene (Rockville, MD, USA).

    Techniques: Staining, Immunohistochemical staining, Expressing, Mutagenesis, Transfection, Western Blot, Produced, Immunofluorescence, Confocal Microscopy, Clone Assay